RESEARCH ARTICLE


Generation of Eosinophils from Unselected Bone Marrow Progenitors: Wild-Type, TLR- and Eosinophil-Deficient Mice



Kimberly D. Dyer, Caroline M. Percopo, Helene F. Rosenberg*
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 186
Abstract HTML Views: 753
PDF Downloads: 215
Total Views/Downloads: 1154
Unique Statistics:

Full-Text HTML Views: 140
Abstract HTML Views: 473
PDF Downloads: 156
Total Views/Downloads: 769



© 2009 Dyer et al;

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA; E-mail: hrosenberg@niaid.nih.gov


Abstract

We have recently devised a culture method that generates large numbers of eosinophils at high purity from unselected BALB/c mouse bone marrow progenitors [Dyer et al., 2008. J. Immunol. 181: 4004-9]. Here we present the extended scope of this approach, as we have used this method successfully to generate eosinophil cultures of virtually 100% purity from bone marrow from C57BL/6 mice, and from TLR2, TLR3, TLR7 and TLR9-gene-deleted mouse strains on the C57BL/6 background. Both wild-type and TLR3 gene-deleted bone marrow eosinophils (bmEos) are functional, releasing peroxidase in response to the secretogogue, platelet activating factor. We have also used this method to re-evaluate production of eosinophils in bone marrow cultures from 􀀂dblGATA mice, a strain that is eosinophildeficient in vivo. Interestingly, bmEos can be detected in the ΔdblGATA cultures (5% of total cells at day 10), although ~80-fold fewer bmEos are detected in 􀀂dblGATA than in parallel wild-type (BALB/c) bone marrow cultures. Overall, we find that generation of large numbers of eosinophils at high purity from unselected bone marrow progenitors proceeds efficiently in a variety of wild-type and gene-deleted strains, and as such this approach shows promise as a universal method for the study of eosinophil structure and function (199 words).

Keywords: Cytokines, Interleukin-5, Toll-Like Receptors, Mouse, Hematopoiesis.