RESEARCH ARTICLE
Pet Induces IL1, TNFα, MIF and IL1Ra Through the IKKαβ/NFκB Pathway
Alejandro Benítez-Guzmán1, Carlos Eslava2, Claudia González-Espinosa3, M. Eugenia Torres-Márquez*, 1
1 Biochemistry and Public Health Departments, School of Medicine. Apdo. Postal 70- 159. 04510 DF, UNAM, México
3 Pharmacobiology Department, Center for Investigation and Advanced Studies, IPN, South Campus, Mexico City, México
Article Information
Identifiers and Pagination:
Year: 2011Volume: 4
First Page: 16
Last Page: 21
Publisher Id: TOIJ-4-16
DOI: 10.2174/1874226201104010016
Article History:
Received Date: 4/6/2011Revision Received Date: 15/6/2011
Acceptance Date: 28/7/2011
Electronic publication date: 19/10/2011
Collection year: 2011
© 2011 Benítez-Guzmán et al;
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Plasmid encoded protein (Pet), a product of E. coli , infiltrates leukocyte in experimental models and acts on macrophages as a chemokine. As an immune response orchestrator, macrophages play an important role in inducing proinflammatory cytokines. In this work, we tested whether in macrophages Pet stimulates the expression of IL1, TNFα, MIF and the antagonist IL1Ra. We also sought the IKKαβ/NFκB pathway as a possible mediator. We found that indeed Pet increases the mRNA of inflammatory cytokines and the antagonist IL1Ra with different rates and kinetics through the IKKαβ/NFκB pathway, which could be consistent as a factor in chronic inflammation.
Keywords: Plasmid Encoded Protein, Inflammation, Signal Transduction, IBD, Macrophage.
